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The disease course and primary CTL responses in naive mice after infection with one of two H9N2 or two H1N1 IAVs.

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posted on 2015-02-10, 02:51 authored by Susu Duan, Victoria A. Meliopoulos, Jennifer L. McClaren, Xi-Zhi J. Guo, Catherine J. Sanders, Heather S. Smallwood, Richard J. Webby, Stacey L. Schultz-Cherry, Peter C. Doherty, Paul G. Thomas

The primary IAV infection was conducted as described in the legends to Fig. 1. (A) Body weight change and (B) virus replication kinetics in mice. (C) The proportion and (D) number of each epitope-specific CTL population, and (E) the total number of the three epitope-specific CTLs in the BAL on d8 or d10. (F) Representative IFN-γ ICS flow cytometry plots from CD8+ T cells in BAL samples. The PB1703, PA224, and NP366 peptide variants specific for each virus were used to stimulate the CTLs to produce IFN-γ for the ICS assay. The data sets represent mean ± SEM; * p<0.05 by Tukey’s test, comparing: (A, B) each virus versus every other virus at that time point, n = 10 per group; (C, D) the indicated epitope versus the other two epitopes in the virus, n = 4–5; (E) the indicated virus versus the other viruses.