The cytoplasmic tail is required for ADAM12L-induced EMT.
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MCF10A cells were infected with lentiviruses expressing either the fusion proteins GFP-ADAM12L, the cytoplasmic domain deletion mutant (GFP-ADAM12L-Δcyto) or the control protein GFP (Control). After antibiotic selection, cells were further enriched using flow cytometry and cultured for 48 hours. (A) Cells were fixed and immunostained for E-cadherin (B) The levels of vimentin and E-cadherin were determined by western blot analyses. Left, representative western blots. Immunoblots for ADAM12 and HSC-70 are shown as control. Right, densitometric analysis of protein amounts. Results are expressed as mean±SD from 3 independent experiments (*, p<0.05).