The anti-proliferation effect of HLC-080 in colon cancer cells
. The time-effect relationship of 2.5 µM HLC-080 and 2.5 µM Sorafenib on proliferation in HT-29 cell line (A).The effect of HLC-080 and Sorafenib on colony formation in human colon cancer cell lines. HT-29 (B), HCT-8 (C) and HCT-116 (D) cells were seeded in a 6-well plate. After 24 h, cells were treated with various concentrations of HLC-080 or Sorafenib. After 14 days of incubation, observe colony size and numbers by Giemsa stain and photograph each well. * P<0.05, ** P<0.01, *** P<0.001, compared with control group.