The accumulation and subcellular redistribution of IL-1β-induced chemokines in HUVECs depends on inhibition of isoprenylation.
HUVECs were pretreated with atorvastatin together with medium (black bars), mevalonate (white bars), squalene (grey bars) or GGPP (hatched bars) before IL-1β-stimulation. In the histogram the four bars of the same color represent cells pretreated with increasing doses of atorvastatin (from the left: 0, 1, 5 and 30 µM). Chemokine levels were measured by ELISA for GRO-α, IL-8, MCP-1 (A) and immunostaining for GRO-α (B). * p<0.05, ** p<0.01 and *** p<0.001 from IL-1β-treated control cells without atorvastatin.