The Zn²⁺ binding site of PyAeADHII.

a) Zn²⁺ binding in the active site of ADH-WT subunit A. The phased anomalous map contoured at 5σ calculated using the Zn-peak data, is shown as blue mesh. b) Metal binding site for Co-substituted PyAeADHII with the cobalt ion drawn as a red sphere. Phased anomalous difference maps contoured at 5σ were calculated using Co-peak data (orange) and Zn²⁺-peak data (blue) and revealed that both ions were present.