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The Osx K58R and K230R mutations enhance the mRNA expression of osteoblast differentiation marker genes.

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posted on 2013-02-19, 15:18 authored by Yanyan Peng, Kaikai Shi, Lintao Wang, Jianlei Lu, Hongwei Li, Shiyang Pan, Changyan Ma

C2C12 cells were transiently transfected with the WT Flag-hOsx plasmid or the K58R and K230R mutants, and 24 h later total RNA was extracted and ALP, Col I and Ocn mRNA were quantified by real-time PCR analysis and normalized to β-actin. Results are mean ± SD of three independent experiments performed in triplicate; *p<0.05, **p<0.01 and ***p<0.001.