The N-terminal 66 residues of Vps74p are not required for cdc34-2-dependent apical growth.
(A) Morphology transformants carrying the plasmid pVT101U (Vec) in cdc34-2 mutant were detected. Transformed cells were grown in a synthetic selection medium at 25°C for 2 h and then shifted to 37°C for 6 h. Next, the ability to complement the elongating morphology was investigated using microscopy. Cells of cdc34-2 with VPS74 deleted were transformed with the indicated plasmids and then imaged as previously described. (B) Yeast strains cdc34-2, pre1-1/pre4-1, cdc53-1, cdc4-, JY25, cdc34-2/vps74Δ, pre1-1/pre4-1/vps74Δ, cdc53-1/vps74Δ, cdc4-/vps74Δ, and JY25/vps74Δ were grown to mid-log phase and these cultures were then transferred from room temperature to 37°C for 6 h and fixed at 37°C. The morphologies of these cells were visualized using microscopy.