Fig_2.tif (837.95 kB)

The Luciferase Immunoprecipitation Systems (LIPS).

Download (0 kB)
figure
posted on 19.03.2015, 03:35 by Shunya Nakane, Osamu Higuchi, Michiaki Koga, Takashi Kanda, Kenya Murata, Takashi Suzuki, Hiroko Kurono, Masanari Kunimoto, Ken-ichi Kaida, Akihiro Mukaino, Waka Sakai, Yasuhiro Maeda, Hidenori Matsuo

The soluble fractionated component from the solubilized HEK 293F cells, including the gAChRα3 or β4-GL, reacted with human serum, and the specific luciferase activities of the gAChRα3 or β4-GL were found with the luminometer. The in vitro LIPS assay can quantitatively evaluate an interaction between an antigen and an antibody with high sensitivity and without a radioisotope.

History