The Luciferase Immunoprecipitation Systems (LIPS).

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posted on 2015-03-19, 03:35 authored by Shunya Nakane, Osamu Higuchi, Michiaki Koga, Takashi Kanda, Kenya Murata, Takashi Suzuki, Hiroko Kurono, Masanari Kunimoto, Ken-ichi Kaida, Akihiro Mukaino, Waka Sakai, Yasuhiro Maeda, Hidenori Matsuo

The soluble fractionated component from the solubilized HEK 293F cells, including the gAChRα3 or β4-GL, reacted with human serum, and the specific luciferase activities of the gAChRα3 or β4-GL were found with the luminometer. The in vitro LIPS assay can quantitatively evaluate an interaction between an antigen and an antibody with high sensitivity and without a radioisotope.

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luciferase immunoprecipitation systems rip Ganglionic Acetylcholine Receptor lips gAChR seronegative group Autoantibodie aag Japanese Patients Autoimmune ganglionic nicotinic acetylcholine receptors seropositive group Autoimmune Autonomic Ganglionopathy

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The Luciferase Immunoprecipitation Systems (LIPS).

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