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The GC-A tryptic peptide phosphorylated at Ser-473 is poorly detected by mass spectrometry.

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posted on 09.05.2012, 00:31 by Andrea R. Yoder, Jerid W. Robinson, Deborah M. Dickey, Joshua Andersland, Beth A. Rose, Matthew D. Stone, Timothy J. Griffin, Lincoln R. Potter

One pmol of synthetic peptides corresponding to tryptic peptides containing phosphorylated versions of Ser-473, Ser-487, or Ser-985 in GC-A were mixed and submitted to nLC-MS-MS. The resulting ion signal intensities are represented above by their individual m/z ion traces on a standard intensity scale. The inset shown for the phosphor-Ser-473 peptide has an expanded y-axis scale.