The Development of Diet-Induced Obesity and Glucose Intolerance in C57Bl/6 Mice on a High-Fat Diet Consists of Distinct Phases - Figure 1
A. Body weight of the HF fed mice differed significantly from those of the LF fed mice from 3 days of feeding (P<0.01) and (P<0.001) onwards. B. The increase in body weight seen in A is mostly fat mass which was increased in the HF mice from 3 days onwards (P<0.001). C. Mean food intake of mice receiving either a LF or HF diet (grams per day). The intake of the HF fed mice was decreased at 3 days on diet (P<0.01) and (P<0.001) thereafter (n = 6–8). D. PF animals showed a decrease in body weight after 3 days on diet compared to LF (P<0.05 and HF fed animals (P<0.001). E. PF animals had a similar body weight to LF fed mice after 1 week on diet. Both LF and PF mice had lower body weight than the HF mice (P<0.001). F. Body fat measured by MRI after 3 days of diet was increased in PF (P<0.05) and HF mice (P<0.001) compared to LF mice despite the lower body weight seen in PF mice in C. G. Body fat after 1 week on diet was higher in both PF (P<0.001) and HF fed mice (P<0.001) compared to LF and was also higher in HF (P<0.001) compared to PF mice. H. Lean body mass after 3 days on diet was similar in all mice LF, PF and HF indicating that all weight changes were due to changes in adiposity. I. Lean mass after 1 week on diet was only increased in HF fed mice compared to LF and PF (P<0.001) (n = 6–8).