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The ABCG2 transporter does not confer resistance to Gemcitabine.

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posted on 2016-02-09, 10:08 authored by Vikash J. Bhagwandin, J. Michael Bishop, Woodring E. Wright, Jerry W. Shay

The dose response of SP cells from (A) H295, (C) Panc-1 monolayer cells, and (E) BxPC3 spheroid cells to vincristine (VCR) and combination of vincristine with verapamil (VCR-VP) was determined using the MTT assay. Similarly, the dose response of SP cells from (B) H295, (D) Panc-1 monolayer cells and (E) BxPC3 spheroid cells to gemcitabine (Gem) and the combination of gemcitabine with verapamil (Gem-VP) was determined using the MTT assay. All data points were generated from a single experiment and represent the mean of six wells per treatment concentration, normalized to the mean of six internal untreated control wells, then transformed by natural log and displayed as a four parameter variable plot as a function of log (drug) versus response. Error bars represent standard error of mean (SEM) from six wells in a single experiment, all of the graphs were generated using Prism statistical analysis software, Student’s t test = P<0.0002.

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