Tfap2a regulates SAG development non-autonomously.
(A-D) Whole-mounts (dorsal up, anterior left) and cross-sections (just posterior to utricular macula, medial to the left) showing both bright-field and corresponding fluorescent images of +/+ host embryos (A, B) and hs:tfap2a host embryos stained for etv5b expression (blue) and showing transplanted wild-type donor cells (red). Positions of wild-type cells that fail to express etv5b are highlighted with arrows in (C, D). (E) Summary of mosaic analysis showing the number of +/+ or hs:tfap2a host embryos examined and the number of +/+ donor cells expressing etv5b over the total number of donor cells that populated the ventral half of the otic vesicle. (F-H) Expression of etv5b at 26 hpf in a control (F), a DM-treated hs:tfap2a embryo (G) and DM-treated wild-type embryo (H). All specimens were treated with 1% DMSO and heat-shocked at 24 hpf. (I-L) Expression of bmp7a at 26 hpf in a control (I, J), tfap2a-/- mutant (K) and hs:tfap2a embryo (L). Wild-type and hs:tfap2a embryos were heat shocked at 24 hpf.