Tetracycline treatment induces detectable apoptosis during spermatogenesis.
TUNEL experiments showing the DNA (PI in red) and incorporated fluorescein-dUTP (green), in samples from un-treated (B, C) or tetracycline-treated (D to F) B. malayi males. (A) Schematic drawing of the male reproductive tract, representing the approximate localization of the different sections observed in the TUNEL assay. (B) Mitotic proliferation zone of spermatogoniae. (C, D) Synaptonemal complexes in meiosis I. (E) Proximal testes. (F) Seminal duct filled with mature spermatocytes. Scale bar = 100 µm.