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Testis of medaka following a 14 day EE2 exposure followed by active breeding with 3 females for 20 days.

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posted on 19.02.2013 by Hilary D. Miller, Bryan W. Clark, David E. Hinton, Andrew Whitehead, Stan Martin, Kevin W. Kwok, Seth W. Kullman

A: DMSO control male. B: Male exposed to 1.0 µg/L EE2. There is thickening of the interstitium, an increase in proportion of spermatocytes, minimal spermatozoa. C: Male exposed to 1.0 µg/L EE2. This organ has severe thickening of the interstitium with a focal area of basophilic cells center of field and a severe decrease in germinal epithelium. D: Male exposed to 10.0 µg/L EE2. Severe thickening of interstitium and severe loss of germinal epithelium are. There are, however, spermatocytes and spermatids present suggesting active spermatogenesis. E: Male exposed to 10.0 µg/L EE2. There is severe thickening of the interstitium with loss of germinal epithelium.

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