Temporal and spatial expression of milk gland protein genes.
(A) Tissue specific RT-PCR. Data represents three replicates. ( B) Time course of mgp1–10 and asmase1 expression during the first two tsetse gonotrophic cycles. Transcript levels were determined by qPCR using a CFX PCR detection system (Bio-Rad, Hercules) and data were analyzed with CFX manager software version 3.1 (Bio-Rad). Data represents the mean ± SE of three replicates and was normalized to tubulin.