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Taste bud cell renewal is inhibited in MRL/lpr mice.

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posted on 19.04.2012 by Agnes Kim, Pu Feng, Tadahiro Ohkuri, Daniel Sauers, Zachary J. Cohn, Jinghua Chai, Theodore Nelson, Alexander A. Bachmanov, Liquan Huang, Hong Wang

(A) BrdU (green) and KCNQ1 (red) double immunostaining of circumvallate papillae from MRL/+/+ and MRL/lpr mice injected with BrdU. Tissues were collected 1 and 5 days after BrdU injection (Day 1 and Day 5, respectively). Arrows denote some BrdU-labeled cells inside taste buds. (B and C) Quantitative analyses of BrdU-labeled cells in the circumvallate (CV) epithelium collected 1 day (B) and 5 days (C) after BrdU injection. Left, number of BrdU-positive taste cells per taste bud profile; right, number of BrdU-positive perigemmal cells per square millimeter (mm2) of circumvallate epithelium. (D) Ki67 (green) and KCNQ1 (red) double immunostaining: merged confocal fluorescent images of circumvallate papillae from MRL/+/+ and MRL/lpr mice. (E) Average number of Ki67-labeled cells in the basal region of taste bud profile. Ki67-labeled cells in the basal regions of circumvallate taste buds were counted, and the average numbers of Ki67-labeled cells per taste bud profile are shown for MRL/+/+ and MRL/lpr mice. (F) Total number of BrdU-positive cells (including cells in the perigemmal regions and inside the taste buds) per square millimeter (mm2) of circumvallate epithelium 1 day after BrdU injection. Four mice per group per time point were included in the experiments. Six circumvallate sections from each animal were included in the analyses. Data are means ± SEM. Student's t tests were used for statistical analysis. * p<0.05; ** p<0.01. Scale bars, 20 µm.

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