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Target region of KRAS gene at codons 12 and 13 for amplification and the design of primers.

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posted on 28.01.2016 by Sirirat Seekhuntod, Paninee Thavarungkul, Nuntaree Chaichanawongsaroj

The positions of primers are illustrated. AS primers share the same forward primer or reverse primer. Solid arrows indicate the forward and reverse primers. The 3′ terminal base of each AS primers was adapted according to its corresponding mutation, and is bold and underlined. Codons and mutated bases are underlined.