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Tandem MS analysis of HeT-A Gag protein by mass spectrophometry.

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posted on 2013-09-18, 02:04 authored by Sukhdev S. Brar, Robert M. Petrovich, Jason G. Williams, James M. Mason

(A) MS/MS of ion m/z shows an extensive y-ion series and the presence of phosphorylation at serines 216 or 221, as indicated by the arrow. (B) Line diagram of different forms of HeT-A Gags (M1, M2, and M3) that were generated by site directed mutagenesis. Primer sets and template used for site directed mutagenesis are described in Table 2.

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