Figure_2.tif (333.91 kB)
Download file

TRAPPC8 interacts mainly with TRAPPC2.

Download (0 kB)
figure
posted on 2011-08-15, 01:35 authored by Min Zong, Xing-gang Wu, Cecilia W. L. Chan, Mei Y. Choi, Hsiao Chang Chan, Julian A. Tanner, Sidney Yu

A. Increasing amounts of GFP-TRAPPC8 cDNA were co-transfected with a constant amount of Myc-TRAPPC2 and Myc-TRAPPC9. The levels of protein expression of all three TRAPP subunits were determined by immunoblotting the lysates of the transfected cells. B. Increasing amounts of GFP-TRAPPC9 cDNA were co-transfected with a constant amount of Myc-TRAPPC2 and Myc-TRAPPC8. C. GFP-tagged TRAPPC8 and the indicated Myc-tagged TRAPPI core subunits were co-transfected into COS cells. Co-IP and subsequent detection by immunoblotting was performed as in Figure 1. The experiments shown are representatives of three independent experiments.

History