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TN sensitivity assay.

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posted on 2011-11-02, 02:34 authored by Lucila I. Buzzi, Sergio H. Simonetta, Armando J. Parodi, Olga A. Castro

F2 Control gfp(RNAi) (A), uggt-1(RNAi) (B) and uggt-2(RNAi) (C) worms were tested under several TN concentrations (0, 2.5, 5 or 10 µg/ml). Gravid hermaphrodites were allowed to lay eggs for 6 h and the developmental stages were analyzed for 5 days at intervals of 24 h and the percentages of L1, L2/L3, L4 and adults were determined. Studies were performed for three independent cohorts (n>100) and the values shown are the mean of the three experiments.

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