TLR agonist treatment induced pro-inflammatory cytokines and NS3 pre-treatment does not induce immune tolerance.
Microglia was exposed to 50 ng/ml of Pam2CSK4 and Pam3CSK4 and pro-inflammatory cytokine expressions were measured by ELISA. (A and B) Pam2CSK4 treatment induced IL-6 and TNF-α production at 3 and 16 hr time point. (C and D) Similarly Pam3CSK4 treatment induced IL-6 and TNF-α production. Microglial cells were pre-treated with 20 ng/ml of NS3 protein for 16 hrs before 6 hr NS3 treatment to study the immune tolerance induction by NS3. (E) There was no significant difference observed for TNF-α production inNS3 exposed and NS3 pre-treated cells. The data is expressed as mean (n = 3) ± SE, *p< 0.05.