TLR9 deficiency improves survival and animal well being in case of severe renal ischemia.
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Renal TLR9 gene expression in wild-type mice at five days following sham operation (sham) (n = 4) or moderate ischemia/reperfusion (IR) (n = 9) (A). Survival of wild-type (Wt) and TLR9 deficient (TLR9KO) mice after five days of severe ischemia/reperfusion (B) (n = 8/group). Wild-type and TLR9KO mice were subjected to sham (n = 7/group) or moderate (20 minutes ischemia) renal injury and assessed for renal function after one day (n = 5–7) and five days (n = 8–9), indicated by plasma urea (C) and plasma creatinine (D). Wild-type and TLR9KO mice were subjected to moderate ischemia (20 minutes) (E) or severe ischemia (30 minutes) (F) followed by one day of reperfusion (n = 7-13/group). During reperfusion, mice were monitored the first 24 hours, every two hours with respect to appearance, behaviour, reactivity and anomalies on a scale of 1 to 5. For every time point, the median is shown. AU = arbitrary units. Statistics were performed using Mann-Whitney where p < 0.01 = **.