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TLR9 deficiency improves survival and animal well being in case of severe renal ischemia.

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posted on 11.09.2015 by Pieter J. Bakker, Angelique M. Scantlebery, Loes M. Butter, Nike Claessen, Gwendoline J. D. Teske, Tom van der Poll, Sandrine Florquin, Jaklien C. Leemans

Renal TLR9 gene expression in wild-type mice at five days following sham operation (sham) (n = 4) or moderate ischemia/reperfusion (IR) (n = 9) (A). Survival of wild-type (Wt) and TLR9 deficient (TLR9KO) mice after five days of severe ischemia/reperfusion (B) (n = 8/group). Wild-type and TLR9KO mice were subjected to sham (n = 7/group) or moderate (20 minutes ischemia) renal injury and assessed for renal function after one day (n = 5–7) and five days (n = 8–9), indicated by plasma urea (C) and plasma creatinine (D). Wild-type and TLR9KO mice were subjected to moderate ischemia (20 minutes) (E) or severe ischemia (30 minutes) (F) followed by one day of reperfusion (n = 7-13/group). During reperfusion, mice were monitored the first 24 hours, every two hours with respect to appearance, behaviour, reactivity and anomalies on a scale of 1 to 5. For every time point, the median is shown. AU = arbitrary units. Statistics were performed using Mann-Whitney where p < 0.01 = **.