TLF surrounds parasites in the PV but is not endocytosed by L. major.
Bone-marrow derived macrophages were infected with GFP-L. major parasites (green) for 2 hours before treatment with prelabelled-Alexa 594-TLF ((10 µg/ml) red) for 2 hours (A) and 24 hours (C). The grey panels are transmission light micrographs of the imaged macrophages. The lack of colocalisation of the two dyes (red and green) is revealed by the 2D cytoflurograms of 25 z-stacks (2 hours, B) and (24 hours, D). Samples were visualized and analyzed with a Leica TCS SP2 AOBS confocal laser scanning microscope.