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TIMP-1-GPI promotes wound healing in vitro.

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posted on 2012-04-27, 01:36 authored by Roghieh Djafarzadeh, Matthias Sauter, Susan Notohamiprodjo, Elfriede Noessner, Pankaj Goyal, Wolfgang Siess, Markus Wörnle, Andrea Ribeiro, Susanne Himmelein, Thomas Sitter, Peter J. Nelson

A monolayer of human primary mesothelial cells was cultured with an experimental wound model insert provided by Ibidi GmbH (Munich, Germany). Removal of the insert yielded a uniform “wound” to the monolayer. (A) Treatment with TIMP-1-GPI but not the control proteins, showed a dose dependent increase in wound closure. (B) The closing areas were calculated using imaging software NIS-Elements. (Video S1, S2, S3, S4, S5).

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