TH phosphorylation evoked by HDZ (10 mg/kg i.p., n = 6) or 2DG (400
mg/kg i.p., n = 5) compared to saline (i.p. n = 6) in the medial prefrontal cortex (mPFC). A) shows schematically the rostrocaudal level and coronal section at the midpoint of tissue extracted for each region examined. The grey shaded regions depict the areas extracted. B) Examples of western blots used for analysis where each lane represents a single animal. Details of analysis are described in the legend for Figure 2. C) In the mPFC 30 min after HDZ a significant increase in pSer31 and pSer19 were evident whereas 30 min after 2DG no changes in phosphorylation were evoked. * P<0.05, ** P<0.01, *** P<0.001.