TGF- receptor inhibitors augment the effects of TKIs on colony growth formation.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Clonogenic assays were performed where cells were treated with DMSO, 0.3μM AZD8010/AZD4547, 0.1μM gefitinib, or both in the presence or absence of a TGF- receptor I (TGFRI) small molecule inhibitor: SB525334 (1μM). After 2 weeks of incubation, colony growth was measured. This assay was performed in a panel of cell lines and is shown here for A. UMSCC25 B. 584-A2 C. UMSCC8 and D. HN31. E. Representative images of the experiment performed with UMSCC8 cells in C. *denotes a p-value <0.05; **denotes a p-value <0.006; *** and ****denotes a p-value <0.0001.