Fig_4.tif (4.55 MB)

TGF- receptor inhibitors augment the effects of TKIs on colony growth formation.

Download (0 kB)
posted on 06.05.2015 by Emily K. Kleczko, Jihye Kim, Stephen B. Keysar, Lydia R. Heasley, Justin R. Eagles, Matthew Simon, Marianne E. Marshall, Katherine R. Singleton, Antonio Jimeno, Aik-Choon Tan, Lynn E. Heasley

Clonogenic assays were performed where cells were treated with DMSO, 0.3μM AZD8010/AZD4547, 0.1μM gefitinib, or both in the presence or absence of a TGF- receptor I (TGFRI) small molecule inhibitor: SB525334 (1μM). After 2 weeks of incubation, colony growth was measured. This assay was performed in a panel of cell lines and is shown here for A. UMSCC25 B. 584-A2 C. UMSCC8 and D. HN31. E. Representative images of the experiment performed with UMSCC8 cells in C. *denotes a p-value <0.05; **denotes a p-value <0.006; *** and ****denotes a p-value <0.0001.