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TBL2 plays an important role in cell growth after exposure to glocose and oxygen deprivation.

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posted on 13.11.2014, 03:35 by Yoshinori Tsukumo, Satomi Tsukahara, Aki Furuno, Shun-ichiro Iemura, Toru Natsume, Akihiro Tomida

(A) TBL2 or PERK shRNA-expressing cells were exposed to glc(−) and/or hypoxia for 12 h and then the cells were reseeded. Relative cell numbers after 3 days were measured by MTT assay. Data is representative of three independent experiments (n = 3) *P<0.05; **P<0.01. (B) Control or TBL2 shRNA-expressing cells were treated with 2 µM thapsigargin for 24 h. Cell viability was calculated by measuring intracellular ATP level. Data is representative of three independent experiments (n = 3) *P<0.05; **P<0.01. (C) The shRNA-expressing cells were exposed to glc(−) and hypoxia for 12 h and were then immediately reseeded. Then the cells were incubated in normal medium for the indicated time (days). Cell numbers at the each time point were counted automatically using a Beckman Coulter Counter. Data is representative of three independent experiments (n = 3) *P<0.05; **P<0.01.

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