TBC-8 localizes to the Golgi-endosomal interface.
(A) The subcellular localization of fluorescently-labeled TBC-8 expressed in motoneurons was analyzed by confocal microscopy. Fluorescently-labeled-TBC-8 localized partially with medial and trans-Golgi markers and showed full co-localization with the early endosomal marker mCherry-RAB-5. No co-localization to RAB-7-positive late endosomes was observed. (B) In motoneurons, mCherry-RAB-2 localizes to the Golgi (left cell body; cell boundary is highlighted by a dashed line). However, when YFP-TBC-8 is overexpressed, mCherry-RAB-2 is redistributed to the cytosol (right cell body). Manns: mannosidase II, TGN: trans-Golgi network. Scale bars represent 3 µm.