T3 uptake by SGHPL-4 cells following MCT8 silencing (A) or MCT8 over-expression (B).
figureposted on 2013-06-12, 02:01 authored by Elisavet Vasilopoulou, Laurence S. Loubière, Heike Heuer, Marija Trajkovic-Arsic, Veerle M. Darras, Theo J. Visser, Gendie E. Lash, Guy S. Whitley, Christopher J. McCabe, Jayne A. Franklyn, Mark D. Kilby, Shiao Y. Chan
SGHPL-4 cells were incubated for 0 to 30 minutes with [125I]-T3. The amount of intracellular radioactivity was quantified and expressed as a percentage of the total radioactivity added. Points represent average of three experiments ±SEM. Within each experiment, each condition was performed in duplicate. Statistically significant differences are indicated by a(control siRNA vs MCT8 siRNA) b(VO vs MCT8), c(VO vs MCT8+ CRYM), d(MCT8+ CRYM vs CRYM) and e(MCT8 vs MCT8+ CRYM); *P<0.05, **P<0.01 and ***P<0.001.