Synaptotagmin-7 staining in cultured hippocampal neurons overlaps with both synaptotagmin-1 and overexpressed SNAP-23/SNAP-25.
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A: Hippocampal neuron overexpressing EGFP-SNAP25 (green channel, upper right) and stained for syt-1 (red, upper left) and syt-7 (blue, lower left). Scale bar = 10 µm. The lower right panel shows the combined image. B: Close-up image shows that syt-1 is concentrated in spots, which represent presynapses, whereas both EGFP-SNAP25 and syt-7 have a more wide-spread distribution. Scale bar = 2 µm. C: Hippocampal neuron overexpressing EGFP-SNAP23 (green channel, upper right) and stained for syt-1 (red, upper left) and syt-7 (blue, lower left). Scale bar = 10 µm. The combined picture is shown in the lower right panel. D: Close-up image shows a widespread distribution of both EGFP-SNAP-23 and syt-7, which overlaps with, but is not restricted to, the areas stained for syt-1. Scale bar = 2 µm.