Survival of the Δppk2 mutant under nutrient, osmotic and aerobic stresses.
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(A) Survival of C. jejuni under nutrient downshift. Survival under nutrient stress was assessed by growing bacterial strains in MEM and determining the CFU at different time points. (B) Survival of C. jejuni under osmotic stress. Survival under osmotic stress in liquid culture was assessed in MH broth containing 0.25 M NaCl. (C) Survival of C. jejuni under osmotic stress in solid medium. Survival in solid medium was determined by spotting 5×102–5×105 CFU onto MH agar containing 0.17 M NaCl. (D) Survival of C. jejuni under aerobic stress. Survival of the C. jejuni 81–176 WT and Δppk2 mutant was assessed by determining the CFU of aerobically grown bacteria at different time points. For all assays, each data point represents the mean±SE of 3 independent experiments. * P≤0.05 and ** P≤0.01.