Suppressors of MPS3-G186K toxicity.
(A) Mutants from the yeast deletion collection were transformed with pURA3-GAL-MPS3-G186K, and transformants were tested for their ability to grow on SC-URA+2% galactose at for 3 d at 30°C. Possible hits were then further examined by western blotting to ensure that Mps3-G186K was expressed at high levels, similar to the wild-type control. Shown here are the hits that both rescued the growth defect of MPS3-G186K and expressed wild-type levels of Mps3-G186K. A full list of genes recovered in this screen is presented in Table S2. Also shown are the wild-type strain and the gal4Δ mutant, which we isolated in the primary screen but eliminated in the secondary screen because it does not express MPS3-G186K. Hits are organized by function using GoSlim analysis. In some cases, we isolated a mutant that deletes part of an overlapping gene, which is indicated below in parenthesis. (B) The indicated genes were deleted in GAL-MPS3-G186K (SLJ1797) and growth was tested in a serial dilution assay at 30°C on YPD (glucose) for 2 d and YPGR (galactose) for 3 d.