Suppression of an amber codon inserted into the GFP-encoding gene.
figure
posted on 2013-02-21, 00:55 authored by Gabrielle Nina Thibodeaux, Xiang Liang, Kathryn Moncivais, Aiko Umeda, Oded Singer, Lital Alfonta, Zhiwen Jonathan Zhang(A) Full-length GFP was expressed in HEK 293T cells only in the presence of a M. jannaschii TyrRS- pair designed to be orthogonal to mammalian cells. (B) Western blot analysis of full-length GFP probed with anti-His antibodies.
