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Suppression of ROS production inhibited nedaplatin-induced cell death.

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posted on 19.08.2015, 02:53 by Zhongyu Liu, Jun Liu, Li Li, Dan Nie, Qilei Tao, Jian Wu, Jiajun Fan, Chen Lin, Shuwei Zhao, Dianwen Ju

(A) HNE1/DDP cells were incubated with varied concentrations of nedaplatin for 12 h. Then, the samples were prepared as described in Materials and Methods. All data are expressed as means ± SD from five independent experiments. *p< 0.05. (B) HNE1/DDP cells were incubated with 6.0 μg/ml nedaplatin in the presence or absence of Baf A1 for 6 h. Then, the cells were treated as described in A. (C) HNE1/DDP cells were treated with 6.0 μg/ml nedaplatin for 12 h in the absence or presence of NAC (10 mM). The cells were treated as described in A. *p<0.05. (D) The LC3 I/II levels were examined by western blot after the nedaplatin treatment with or without of NAC (10 mM) for 48 h. (E) HNE1/DDP cells were treated with 6.0 μg/ml nedaplatin for 48 h in the absence or presence of NAC (10 mM). The cell viability was determined as described in Materials and Methods. Data are mean ± SD from five independent experiments. ***p<0.001 compared to nedaplatin only.

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