Subgroups have distinct patterns of expression for PI3K/AKT/mTOR pathway components.
Tumors (x axis) were grouped by AKT class then Z transformed mRNA (A) or protein and phospho-protein expression (B) color coded to reflect magnitude (y axis). The Pearson correlation coefficient for AKT pS473 vs. RPS6 pS235/236 (light gray) and AKT pS473 vs. RPS6 pS240/244 (dark gray) for each subgroup is shown (C). Proposed AKT/mTOR/S6 pathway map for the MES and SL subtypes based on this data (D). This model shows loss of AKT and mTOR inhibitors (PHLPP, TSC and pAMPK) increases output of the AKT/mTOR/S6 axis (pRPS6) in the MES subgroup. Conversely, increased expression of these inhibitors decreases output in the SL subgroup. Red, grey and green represent high, intermediate and low expression/activity, respectively.