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Structure of the anti-TssM nanobodies.

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posted on 26.03.2015 by Van Son Nguyen, Laureen Logger, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, Badreddine Douzi, Eric Durand, Alain Roussel, Eric Cascales, Christian Cambillau

(A) Sequence alignment of nanobodies nb02, nb25 and nb42. Identical amino-acids are in white and underlined in red, similar amino-acids are colored in red, different amino-acids are in black. The CDR1, CDR2 and CDR3 are highlighted in blue, green and yellow, respectively. The green numbers (1 or 2) below the sequence indicate cysteine residues involved in disulphide bridges SS1 (1, Cys22 and Cys96) and SS2 (2, Cys50 and Cys106) disulphide bridges formation. Crystal structure of nanobodies nb02 (B) and nb25 (C), represented as ribbons and colored in rainbow mode. (D) Superimposition of the structures of nb02 (grey) and nb25 (rainbow color). The locations of the CDR1, CDR2 and CDR3 variable regions are indicated as well as the positions of disulphide bridges (SS1 and SS2).