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Stereological Assessment of PC Density.

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posted on 24.02.2014, 03:29 by Jerry Skefos, Christopher Cummings, Katelyn Enzer, Jarrod Holiday, Katrina Weed, Ezra Levy, Tarik Yuce, Thomas Kemper, Margaret Bauman

This figure depicts our stereological approach for quantifying PC density in each region of the cerebellum. 2a displays a full sagittal section of the human brain. 2b represents the positioning of contour lines used to select the boundaries around each region of interest, within which sampling occurs. Lobules IV–VI are highlighted in green, crus I is highlighted in pink, crus II is highlighted in blue, and the flocculus of lobule X is highlighted in orange at the bottom of the section. 2c displays a hypothetical placement of counting frames (optical disector probes) within lobules IV–VI. The counting frame size has been increased to aid in visualization for this example. The white dotted line displays the randomly imposed grid over the contour, which regulates the distance between counting frames along the X–Y plane of the section. A counting frame is placed in the top-left of each grid cell if it will include a portion of the region of interest as designated by the contour line. Finally, 2d displays a histological section with an imposed counting frame that includes two PCs to be counted. The use of the optical disector probe dictates that PCs are counted when within the counting frame or at all touching the green line, but never when cells intersect with the red line.