Figure_6.tif (163.06 kB)

Status of the Treg-specific demethylated region (TSDR) predicts functional activity.

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posted on 20.02.2013 by Tatiana N. Golovina, Tatiana Mikheeva, Todd M. Brusko, Bruce R. Blazar, Jeffrey A. Bluestone, James L. Riley

Prior to setting up the suppression assays displayed in Fig 5, 1 million expanded T cells were harvested, genomic DNA was prepared, and methylation status of TSDR was determined as described in the Material and Methods. The percentage of demethlyated CpG residues from two donors is displayed. R, A, and RA refers to Tregs expanded in RAPA, ATRA or RAPA and ATRA respectively.