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Standard curve of GFP fluorescent signal from lysed GFP-MSCs.

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posted on 20.02.2013, 02:37 by Matthew C. Phipps, Yuanyuan Xu, Susan L. Bellis

GFP-MSCs were counted using a hemocytometer and set numbers of cells were spun down in a centrifuge. Cell pellets were lysed and solution fluorescence was measured by a fluorometer. The coefficient of determination for the linear regression was 0.999, showing a very strong linear correlation between GFP-MSC number and solution fluorescence.