Splitting clusters of objects.
(A) Thresholding this image of two nuclei results in one continuous outline rather than two objects. Original image from project described in Moffat et al. . (B) The local maxima in the smoothed image correspond poorly with the centers of the nuclei. (C) The local maxima (red squares) in the distance transform of the image (shown as contours) correspond well with the centers of the nuclei. (D) Seeded watershed from the local maxima in (C) divides the cluster correctly.