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Spatiotemporal distribution of TH in E11 to E15 rat embryos.

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posted on 2013-02-20, 10:47 authored by Sophie Croizier, Clotilde Amiot, Xiaoping Chen, Françoise Presse, Jean-Louis Nahon, Jane Y. Wu, Dominique Fellmann, Pierre-Yves Risold

(A–D) Photomicrographs of para-sagittal sections through the diencephalon of E13 (A, B), E14 (C) and E15 (D) rat embryonic brains to illustrate the distribution of tyrosine hydroxylase (TH) immunofluorescence signal. At E13, cells (arrows in A and B) are labeled caudal to the optic stalk (os) and axons course in the direction of the midbrain through the tpoc. In the ventral midbrain, some cells are also detected (DA), but the mesotelencephalic tract is not yet visible. At E14 and E15, although cells and axons are still visible in the retrochiasmatic region, the mesotelencephalic axons form a prominent tract which first follows up the tpoc, and take a rostral route towards the telencephalon at the level of the caudal hypothalamus (HYP). (E, F) Photomicrograph of a para-sagittal E15 rat brain section labeled using immunoperoxidase to detect MCH (E) and immunofluorescence for TH (F). MCH cell bodies are within or close to the dopaminergic mesotelencephalic tract. See text for details. Scale Bar: A–D = 250 µm; E, F = 50 µm.

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