Sonicated M.fermentans Protein inhibits the DNA relaxation activity of Topo I.
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MCF7 (A–C) and U251 (D–F) cancer cells were treated for 24 hrs with various concentrations of M. fermentans proteins. Total nuclear proteins (12.5 ng) were added to a specific reaction mixture for Topo I. Reaction products were analyzed by agarose gel electrophoresis. (A,D) A representative picture (n = 4–5) of Topo I DNA relaxation activity. (B,E) Quantification analysis of Topo I activity. (C,F) Topo I protein level examined by Western blot analysis. Symbols: R and S are the relaxed and supercoiled form of the pUC19 DNA, respectively, Topo- no protein added to the reaction mixture. t-test: *p<0.05, **p<0.01, ***p<0.005.