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Sonicated M.fermentans Protein inhibits the DNA relaxation activity of Topo I.

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posted on 27.08.2013 by Reuven Afriat, Shulamith Horowitz, Esther Priel

MCF7 (A–C) and U251 (D–F) cancer cells were treated for 24 hrs with various concentrations of M. fermentans proteins. Total nuclear proteins (12.5 ng) were added to a specific reaction mixture for Topo I. Reaction products were analyzed by agarose gel electrophoresis. (A,D) A representative picture (n = 4–5) of Topo I DNA relaxation activity. (B,E) Quantification analysis of Topo I activity. (C,F) Topo I protein level examined by Western blot analysis. Symbols: R and S are the relaxed and supercoiled form of the pUC19 DNA, respectively, Topo- no protein added to the reaction mixture. t-test: *p<0.05, **p<0.01, ***p<0.005.