Sonic Hedgehog expression decreases and vitronectin expression increases during development.
A and B. Temporal expression of vitronectin, ep300, and Shh mRNAs in cerebellar cortices enriched in Purkinje cells by Affymetrix (A) and qPCR (B) during the first 10 postnatal days. Values for P3, P5, P7, and P10 are expressed as a percentage of P0 values. Note the opposing developmental changes in the expression of Shh and VN, whereas the expression of ep300 does not vary. C. Expressions of Shh and vitronectin by RT-qPCR from L7-GFP purified PCs on a FACsAria apparatus during the first 10 postnatal days. Values for P0 and P3, and P7 and P10 are pooled respectively; P7–P10 values are expressed as a percentage of P0–P3 values.