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Somatostatin Analogues for Receptor Targeted Photodynamic Therapy - Figure 4

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posted on 11.08.2014, 03:03 authored by Slávka Kaščáková, Leo J. Hofland, Henriette S. De Bruijn, Yunpeng Ye, Samuel Achilefu, Katy van der Wansem, Angelique van der Ploeg-van den Heuvel, Peter M. van Koetsveld, Michael P. Brugts, Aart-Jan van der Lelij, Henricus J. C. M. Sterenborg, Timo L. M. ten Hagen, Dominic J. Robinson, Martin P. van Hagen

(A) PDT effects of un-conjugated Ce6 and conjugate 1 (c = 1 µM) on K562 sst2+ cell viability as a function of light dose. There is a statistically significant difference between the viability of cells incubated with un-conjugated Ce6 followed by irradiation with different light doses and the non-irradiated cells (groups of controls) (p<0.005). In case of conjugate 1, only 5−2 light dose shows the significant difference (p<0.005) when compare to its control (e.g. non-irradiated cells incubated with conjugate 1); (B) Confocal fluorescence images of un-conjugated Ce6 in K562 sst2+ cells after 15 min and 4-h incubation in serum-free medium; (C) Confocal fluorescence images of conjugate 1 after 15 min (top left image) and 4-h incubation (top right image) in serum-free medium. Bottom right image shows conjugate 1 localization 4-h after incubation and its corresponding transmission image (bottom left).