SmInAct Is a Member of the TGF-β/Activin Subfamily
(A) ClustalW alignment of the C-terminal domain of the SmInAct protein with three other members of the TGF-β/Activin subfamily. Amino acids identical to the SmInAct sequence are shaded gray. Numbers to the right indicate position of the last amino acid in the row within each respective full-length sequence. Stars indicate invariant amino acid residues in TGF-β homologs.
(B) Phylogenetic dendrogram demonstrating that SmInAct is a member of the TGF-β superfamily. SmInAct (red) is shown clustering among members of the TGF-β/Activin subfamily (solid line), and not with members of the BMP/growth differentiation factor subfamily (dashed line). Conserved residues in the C-terminal region of each homolog (final 94–106 amino acids) were used in the analysis. Percentages at branch points are based on 1,000 bootstrap runs.