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Single and double transformation frequency in wildtype, ssbB, and ssbBΔ7 cells.

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posted on 30.06.2011, 02:44 by Laetitia Attaiech, Audrey Olivier, Isabelle Mortier-Barrière, Anne-Lise Soulet, Chantal Granadel, Bernard Martin, Patrice Polard, Jean-Pierre Claverys

(A) Competent cells (∼3.0 108 cfu ml−1) of strain R1818 (ssbB+), R2647 (ssbBΔ7) and R2646 (ssbB) were exposed to various concentrations of R304 chromosomal DNA and SmR transformants were scored. Data represent the compilation of six independent experiments shown in Figure S3 (left panel). Bottom panel: ratio between the number of SmR transformants in ssbB mutant and wildtype cells calculated from data in top panel. (B) Same conditions as in panel A but scoring for RifR SmR double transformants. Data represent the compilation of four independent experiments shown in Figure S3 (rigth panel). Bottom panel: ratio between the number of RifR SmR transformants in ssbB mutant and wildtype cells calculated from data in top panel.

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