Simultaneous morphology and caspase-3/7 imaging.

(A) Superimposed time-lapsed images showing the morphological change and caspase-3/7 activation in two individual differentiated RGC-5 cells (cell #1 and cell #2) going through apoptosis under 100 mmHg over 20 hours. The images are composed by overlapping phase-contrast image and red fluorescence image of cells loaded with MR-(DEVD)₂. Plots for MR-(DEVD)₂ fluorescence intensity, neurites number, and cell body area for cell #1 (B) and #2 (C) shown in (A).