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Simultaneous morphology and caspase-3/7 imaging.

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posted on 18.10.2010, 01:16 by Jae Kyoo Lee, Siyuan Lu, Anupam Madhukar

(A) Superimposed time-lapsed images showing the morphological change and caspase-3/7 activation in two individual differentiated RGC-5 cells (cell #1 and cell #2) going through apoptosis under 100 mmHg over 20 hours. The images are composed by overlapping phase-contrast image and red fluorescence image of cells loaded with MR-(DEVD)2. Plots for MR-(DEVD)2 fluorescence intensity, neurites number, and cell body area for cell #1 (B) and #2 (C) shown in (A).