Figure_5.tif (1.12 MB)
Download file

Silencing CRACC expression on Kupffer cells alleviates the liver injury induced by Poly I:C/D-GalN.

Download (0 kB)
figure
posted on 30.09.2013, 02:37 by Yangxi Li, Guoshuai Cao, Xiaodong Zheng, Jun Wang, Haiming Wei, Zhigang Tian, Rui Sun

A-C, Mice were treated with nanoparticle encapsulated FAM conjunct siRNA by intravenous injection; and 3h later FAM+ cells among Kupffer cells were analyzed by flow cytometry (A); and the nanoparticle encapsulated FAM conjunct siRNA (green) endocytosed by Kupffer cells (red, stained with APC-F4/80 mAb) were tracked by con-focal microscopy both in isolated Kupffer cells (B, Bar=50µm, 63× objective) and in site of frozen tissue sections(C, Bar=50µm, 40× objective). Kupffer cells were identified as F4/80+ cells. D, Mice were pre-treated with nanoparticle encapsulated siNeg or siCRACC for 6h, and then treated with Poly I:C/D-GalN in each group. The CRACC expression on Kupffer cells was analyzed by flow cytometry at 12h and 18h time points post the Poly I:C/D-GalN treatment. The Data are from 6-8 mice per group. E-F, The mice were pre-treated with nanoparticle encapsulated siNeg or siCRACC for 6h, and then treated with Poly I:C/D-GalN in each group. The serum ALT was tested (E) and the H&E-Staining analyses were performed at 18h time point post Poly I:C/D-GalN (F). The Data are from 6-8 mice per group. **P < 0.01.

History

Usage metrics

Categories

Licence

Exports