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Serum Processing and Associated Particle Content.

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posted on 07.06.2013 by Heather H. Cheng, Hye Son Yi, Yeonju Kim, Evan M. Kroh, Jason W. Chien, Keith D. Eaton, Marc T. Goodman, Jonathan F. Tait, Muneesh Tewari, Colin C. Pritchard

(A) Left: Schematic of SerumSTD processed with a 0.22 um filtration step to form SerumFILT. Right: Histogram content of platelets and microparticles in stepwise processed plasma samples. X-axis is particle diameter and Y-axis is number of particles/mL. (B) Platelet and microparticle content (in thousand/µL) of serum samples as measured by particle counter and hematology analyzer. (B) Validation of selected miRNAs by individual qRT-PCR assay. Normalized CT difference from mean of all 7 samples (including 5 plasma samples) is shown. MicroRNAs are arranged as in Figure 3. Average fold difference between SerumSTD vs. SerumFILT is indicated below.