Sequencing and qRT-PCR.

Quantitative RT-PCR validation of differentially expressed genes in control (a basal diet) and treatment (diet with 120 mg/kg ASE) samples from liver and ovary tissues, including eight genes. All data were normalized to the expression level of actin. Data represent fold change of relative quantification in treatment vs. control samples. The error bars represent the range of the fold change as determined by the data assist software.